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  Indian J Med Microbiol
 

Figure 1: Scatter plot: Testing of the phenylalanine hydroxylase gene E280K mutation with hydrolysis probe allele discrimination assays with automatic allele calling. Genotyping plot of 15 clinical samples and nontemplate control; homozygous wild-type (E280K WT) alleles (◆), homozygous mutant alleles (E280K Mut) (●), heterozygote allele (E280K Hetero) (■), and nontemplate control (▼); all genotypes were also correctly identified by the high-resolution melting method (see below); the FAM fluorescence, which indicates the degradation of the wild-type E280K probe (Y axes), is plotted against the HEX fluorescence, which indicates the degradation of the mutant E280K probe (X axes) using the baseline-corrected fluorescence (dR)

Figure 1: Scatter plot: Testing of the phenylalanine hydroxylase gene E280K mutation with hydrolysis probe allele discrimination assays with automatic allele calling. Genotyping plot of 15 clinical samples and nontemplate control; homozygous wild-type (E280K WT) alleles (◆), homozygous mutant alleles (E280K Mut) (●), heterozygote allele (E280K Hetero) (■), and nontemplate control (▼); all genotypes were also correctly identified by the high-resolution melting method (see below); the FAM fluorescence, which indicates the degradation of the wild-type E280K probe (Y axes), is plotted against the HEX fluorescence, which indicates the degradation of the mutant E280K probe (X axes) using the baseline-corrected fluorescence (dR)